Evaluation of antibacterial activity of probiotic Bifidobacterium longum and Saccharomyces cerevisiae against E. coli O157:H7

Abstract

This research was directed to assess the antibacterial activity of the probiotic strains Bifidobacterium longum and Saccharomyces cerevisiae against Escherichia coli O157:H7, a pathogenic strain responsible for severe foodborne illness. The study employed three methods spot method, agar diffusion and vertical line method to evaluate the antibacterial action of B. longum and S. cerevisiae. Bifidobacterium longum exhibited considerable antibacterial activity, with noticeable inhibition of E. coli H7: O157 growth in comparison to S. cerevisiae. Despite the fact that S. cerevisiae showed antibacterial properties, its inhibition activity was not as much as than those of B. longum. The study asserts that Bifidobacterium longum showed a remarkable antibacterial effect against E. coli H7: O157, proposed its suggesting probable application as an agent to prohibit or control this pathogen infection. Saccharomyces cerevisiae additionally displayed antibacterial effects, although it is less efficient. However, the combination of both of them exhibited remarkable inhibition. These findings will be paved the way of understanding the influence of probiotics in infection control and food safety.