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Association of MTHFR gene polymorphism (rs1801133) with Type 2 Diabetic Iraqi population

Salih M. Alkhafaji
Anwar M. Aljanabi
Journal of Progressive Medical Sciences, Vol. 1 No. 2 (2025): Journal of Progressive Medical Sciences
Published: 2025-12-06

Abstract

Background: Type 2 Diabetes Mellitus is a complex of endocrine metabolic disorder. It is believed that polymorphism of Methylenetetrahydrofolate reductase (MTHFR) C677T related to type 2 diabetes mellitus. However, results are conflicted from different ethnic and races. This study aimed to evaluate the relationship between MTHFR (rs1801133) gene polymorphism and type 2 diabetes mellitus.


Methods:  This case-control study was included 100 patients were diagnosed with type 2 diabetes mellitus (T2DM) cases and 100 healthy control individuals. The blood sample was collected for estimation of biochemical parameters for analysis among the T2DM cases and healthy control groups. DNA extraction from whole blood was done to study the MTHFR gene polymorphism by RFLP-PCR method.


Results: There were significant difference in genotype distribution among Type2 diabetic patients and control group. Compared with CC wild genotype, CT heterozygous genotype (OR= 5.7, 95% Cl= 3.0-11.0 and P=0.0001) and TT homozygous genotype (OR=4.3, CI=1.5-11.9 and P=0.0005). The T allele frequency increased the risk in diabetic patients by three folds when compared with C allele (OR= 3.6, C. I=2.2-5.5, P= 0.0001), suggested that the effect of MTHFR point mutation on type 2 diabetes mellitus implicated with increased risk of disease.


Conclusion; Our results indicate that polymorphism in MTHFR C677T plays significant role in type II diabetes mellitus risk for Iraqi populations

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Author Biography

Salih M. Alkhafaji
None
Anwar M. Aljanabi
None
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