Evaluation of the Efficacy of Carpesium Cernuum leaves nanoparticles on Apoptotic Pathway in MNU-induced Brain injury in Male Albino Rats
Efficacy of Carpesium Cernuum leaves nanoparticles
DOI:
https://doi.org/10.63939/ah3t4r02Keywords:
Carpesium cernuum, nanoparticles, N-Methyl-N-nitrosourea (MNU), apoptosis, oxidative stress, inflammation, neuroprotection, male albino ratsAbstract
Background: N-methyl-N-nitrosourea (MNU) is an alkylating reagent that causes oxidative stress, inflammation and disruption of apoptotic signaling leading to cell injury. Carpesium cernuum possesses bioactive phytochemical constituents that may possess antioxidant and anti-inflammatory activities. The present study aims, to investigate the protective effects of nano-formulated Carpesium cernuum leaf extract on 30 mg/Kg b.w. of MNU-induced brain injury in male albino rats affecting apoptotic, inflammatory and oxidative stress parameters and histological changes. Methodology: The objective of this experiment was to test the effectiveness of Nano-Carpesium cernuum leaf extract on regulating the apoptotic process in the brain tissues of male albino rats induced with N-methyl-N-nitrosourea (MNU). Forty male albino rats were arbitrarily assigned into four groups: n = 10/group) , one group being the control group, another MNU-induced group, and the last Nano-Carpesium cernuum leaf extract with MNU group. “At the end of the experimental period, blood samples were collected to determine the levels of BCL-2, Caspase-3, Caspase-8, CEA, TNF-α, IL-6, IL-1β, COX, COX-2, and the antioxidant enzyme SOD. Brain tissues were also examined grossly and histopathologically. Results: The results demonstrated that MNU exposure significantly decreased BCL-2 and SOD levels, while significantly increasing Caspase-8, CEA, TNF-α, IL-6, IL-1β, and COX-2, indicating activation of apoptosis, oxidative stress, and inflammatory responses”. Histopathological examination revealed inflammatory cell infiltration, spongiform changes, and liquefactive necrosis in brain tissue. In contrast, treatment with Nano-Carpesium cernuum Leaf Extract + MNU significantly improved apoptotic, inflammatory, and oxidative stress biomarkers and markedly reduced histopathological brain damage. Conclusions: These findings suggest that the nano formulation of Carpesium cernuum leaf extract support the potential neuroprotective activity, antioxidant, anti-inflammatory, and anti-apoptotic properties against MNU-induced brain injury in male albino rats.
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References
1. Shi, N. N., et al. (2022). Carpesium genus constituents. Heterocyclic Communications, 28(1), 95–123
2. Zhang, T., Zhang, Q. B., Fu, L., Li, L. Y., Ma, L. Y., Si, J. G., ... & Zou, Z. M. (2019). New antiproliferative germacranolides from Carpesium divaricatum. RSC advances, 9(20), 11493-11502.
3. Diek, D., Smidt, M. P., & Mesman, S. (2022). Molecular organization and patterning of the medulla oblongata in health and disease. International Journal of Molecular Sciences, 23(16), 9260.
4. Nóbrega, C., Nascimento-Ferreira, I., Onofre, I., Albuquerque, D., Hirai, H., Déglon, N., & de Almeida, L. P. (2013). Silencing mutant ataxin-3 rescues motor deficits and neuropathology in Machado-Joseph disease transgenic mice. PloS one, 8(1), e52396.
5. Van Opdenbosch, N., & Lamkanfi, M. (2019). Caspases in cell death, inflammation, and disease. Immunity, 50(6), 1352-1364.
6. Hao, C., Zhang, G., & Zhang, L. (2019). Serum CEA levels in 49 different types of cancer and noncancer diseases. Progress in molecular biology and translational science, 162, 213-227.
7. Silva, F. F. V. E., Padín-Iruegas, M. E., Caponio, V. C. A., Lorenzo-Pouso, A. I., Saavedra-Nieves, P., Chamorro-Petronacci, C. M., … & Pérez-Sayáns, M. (2022). Caspase 3 and cleaved caspase 3 expression in tumorogenesis and its correlations with prognosis in head and neck cancer: a systematic review and meta-analysis. International journal of molecular sciences, 23(19), 11937.
8. Takahashi, S. (2022). Metabolic contribution and cerebral blood flow regulation by astrocytes in the neurovascular unit. Cells, 11(5), 813.
9. Coulombe, V., Saikali, S., Goetz, L., Takech, M. A., Philippe, É., Parent, A., & Parent, M. (2020). A topographic atlas of the human brainstem in the ponto-mesencephalic junction. Brain Structure and Function, 225(5), 1435–1448
10. Mercadante, A. A., & Tadi, P. (2023). Neuroanatomy, gray matter. StatPearls Publishing. https://www.ncbi.nlm.nih.gov/books/NBK554494/
11. Banerjee, S., Nau, S., Hochwald, S. N., Xie, H., & Zhang, J. (2023). Anticancer properties and mechanisms of botanical derivatives. Phytomedicine Plus, 3(1), 100396.
12. Swanson, L. W., Hahn, J. D., & Sporns, O. (2021). Subsystem macroarchitecture of the intrinsic midbrain neural network and its tectal and tegmental subnetworks. Proceedings of the National Academy of Sciences, 118(20), e2101869118.
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